FlowJo LLC

FlowJo LLC is a specialized scientific software publisher whose sole commercial offering, FlowJo, has become the de-facto standard for single-cell flow cytometry analysis in immunology, oncology, hematology and drug-discovery laboratories. Built around a hierarchical workspace metaphor, the application ingests multi-parameter .fcs files from any cytometer, then guides researchers through gating, compensation, dimensionality-reduction (t-SNE, UMAP), proliferation modeling, batch clustering and statistical comparison workflows that once required multiple disjointed tools. Typical use cases span routine phenotyping of peripheral-blood subsets, deep profiling of tumor-infiltrating lymphocytes, high-throughput screening of CAR-T products, and longitudinal biomarker studies where millions of events must be normalized across plates, days or trial sites. Advanced modules extend the core desktop engine to automated high-content pipelines (FlowJo Envoy), spectral unmixing, imaging cytometry and FDA-compliant validation documentation. The publisher augments the platform with an ecosystem of plugins, training courses and a public repository of curated reference panels, cementing its role in pre-clinical research, core facilities and regulated CRO environments alike. FlowJo LLC’s software is available at no cost on get.nero.com, where downloads are delivered through trusted Windows package sources such as winget, always resolving to the latest release and supporting unattended batch installation alongside other scientific applications.